Journal: Applied and Environmental Microbiology
Article Title: Screening of Chlamydomonas reinhardtii Populations with Single-Cell Resolution by Using a High-Throughput Microscale Sample Preparation for Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
doi: 10.1128/AEM.01201-15
Figure Lengend Snippet: High-throughput microscale sample preparation for single-cell MALDI-MS. (A to D) Schematics of the individual steps of the workflow. (E to H) Confocal fluorescence scans of 9 wells on the microarray. (A, E) The cleaned, empty microarray slide has wells where the stainless steel surface is roughened up. (B, F) Cells are spotted from cell suspensions. Chlorophyll autofluorescence makes the cells clearly visible. (C, G) Fast freezing of the cells in liquid nitrogen leads to cell wall damage. (D, H) Chlorophyll and other soluble analytes are extracted from the cells by the matrix solution (10 mg/ml DHB in aqueous acetone). Evaporation of the solvent leads to cocrystallization of the extracted analytes with the MALDI matrix.
Article Snippet: After spotting the cells, the slide was scanned using a confocal microarray scanner (LS400; Tecan).
Techniques: High Throughput Screening Assay, Sample Prep, Fluorescence, Microarray, Evaporation, Solvent